Erratum to: Stability of operational taxonomic units: an important but neglected property for analyzing microbial diversity
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منابع مشابه
Erratum to: Stability of operational taxonomic units: an important but neglected property for analyzing microbial diversity
[This corrects the article DOI: 10.1186/s40168-015-0081-x.].
متن کاملStability of operational taxonomic units: an important but neglected property for analyzing microbial diversity
BACKGROUND The operational taxonomic unit (OTU) is widely used in microbial ecology. Reproducibility in microbial ecology research depends on the reliability of OTU-based 16S ribosomal subunit RNA (rRNA) analyses. RESULTS Here, we report that many hierarchical and greedy clustering methods produce unstable OTUs, with membership that depends on the number of sequences clustered. If OTUs are re...
متن کاملDefining DNA-based operational taxonomic units for microbial-eukaryote ecology.
DNA sequence information has increasingly been used in ecological research on microbial eukaryotes. Sequence-based approaches have included studies of the total diversity of selected ecosystems, studies of the autecology of ecologically relevant species, and identification and enumeration of species of interest for human health. It is still uncommon, however, to delineate protistan species base...
متن کاملQuantitative community fingerprinting methods for estimating the abundance of operational taxonomic units in natural microbial communities.
Molecular fingerprinting techniques offer great promise for analyzing changes in microbial community structure, especially when dealing with large number of samples. However, a serious limitation has been the lack of quantification offered by such techniques since the relative abundances of the identified operational taxonomic units (OTUs) in the original samples are not measured. A quantitativ...
متن کاملDelimiting operational taxonomic units for assessing ciliate environmental diversity using small-subunit rRNA gene sequences.
Delineating operational taxonomic units (OTUs) is a central element in any culture-independent analysis of environmental microbial eukaryotic diversity. Previous studies either have not justified their choice in sequence distance used to bin small-subunit ribosomal RNA (SSU rRNA) gene sequences amplified from environmental samples into OTUs, or have used a value based on the average across a br...
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ژورنال
عنوان ژورنال: Microbiome
سال: 2015
ISSN: 2049-2618
DOI: 10.1186/s40168-015-0098-1